iDx LegioTest 100

Fast track detection of Legionella pneumophila SG 1 

The iDx LegioTest 100  is a quantitative fast track test for Legionella pneumophila SG 1.

The detection of Legionella on an indirect enzyme-linked immunosorbent assay (ELISA) using specific polyclonal antibodies against Legionella. The sample preparation and result detection are a fully automated process inside the iDx BenchTop to ensure a hygienic handling of pathogens.

Product Highlights


Fast track detection of Legionella in drinking water

Fast: < 1 h from sample application to result presentatio

Handsfree system due to innovative microfluidic biosensor technology and cartridge-based liquid handling

High reliability due to a full automated process from sample preparation to detection

No additional lab equipment or laboratory skills necessary

Full iDx cloud platform enabling data collection, analytics, device maintenance and compliance

The iDx cloud is a comprehensive platform using fully encrypted data traffic accordingly European guidelines. The intelligent platform empowers a digital device management, data storage and varied data analytics via intuitive graphical dashboards. All test results can be used every time, everywhere on every iDx smart device.

Parameters of the Test System


Detected bacteria

Legionella pneumophila SG1 (serogroup 1; WDCM 00107; ATCCTM 33152)


detection limit <100 CFU/100ml


 < 1 h from sample application to result presentation

Method of detection

indirect enzyme-linked immunosorbent assay (ELISA)

No cross-reaction with

Pseudomonas aeruginosa, Serratia marcescens, Acinetobacter haemolyticus, Escherichia coli



Legionella are rod-shaped bacteria living in water, which in 90% of cases are the cause of legionellosis or Legionnaires’ disease. Currently, many species of Legionella have been described, all of which have pathogenic effects on humans. The best known and most widespread is Legionella pneumophila.

Source: Doccheck. Flexikon. (2021, June 3). Legionellen.

Rust, lime deposits or other sediments serve as a source of nutrients and as protection, thus providing optimal conditions for rapid multiplication. Legionella find ideal growth conditions at temperatures between 25 and 50°C; at water temperatures above 55°C, Legionella growth is effectively inhibited until, above 60°C, the germs finally die off.

Under certain conditions, industrial plants such as evaporative cooling systems, cooling towers and wet separators can emit aerosols containing Legionella into the outside air, which can lead to severe pneumonia and even death if inhaled.

Source: Bundeszentrale für gesundheitliche Aufklärung (2021, June 3). LEGIONELLEN. Informationen über Krankheitserreger beim Menschen.

In 1976, after a congress in a large hotel in Philadelphia, 220 out of 4000 participants fell ill, 34 of them died of a previously unknown pneumonia caused by a bacterium. Since those who fell ill were members of the American Legion, the bacterium was christened Legionella pneumophila and the disease was called Legionnaires’ disease.

Source: Doccheck. Flexikon. (2021, June 3). Legionellen.

The Robert Koch Institute is the German government’s central institution for the identification, surveillance and prevention of infectious diseases in the Portfolio of the German Ministry of Health.

Within the framework of the Infection Protection Act, the Robert Koch Institute has the responsibility to collect the data of notifiable diseases sent to it and to evaluate them from an infection epidemiological point of view.

Source: Robert Koch-Institut (2021, June 3). LEGIONELLEN. Informationen über Krankheitserreger beim Menschen.